Fusion proteins of Arabidopsis cap-binding proteins: Cautionary “tails” of woe
نویسندگان
چکیده
منابع مشابه
Distinct Features of Cap Binding by eIF4E1b Proteins
eIF4E1b, closely related to the canonical translation initiation factor 4E (eIF4E1a), cap-binding protein is highly expressed in mouse, Xenopus and zebrafish oocytes. We have previously characterized eIF4E1b as a component of the CPEB mRNP translation repressor complex along with the eIF4E-binding protein 4E-Transporter, the Xp54/DDX6 RNA helicase and additional RNA-binding proteins. eIF4E1b ex...
متن کاملusing rice flour for purification of maltose binding fusion proteins expressed in escherichia coli
background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...
متن کاملfunctional study of p0 proteins of two cereal yellow dwarf viruses (cydv-rpv and cydv-rps) and identification of their cellular partner
نقش سرکوبگری پروتئین p0 در دو پولروویروس کوتولگی زردی غلات cydv-rpv) و (cydv-rps، متفاوت در شدت بیماریزایی، مورد مطالعه قرار گرفت. نتایج نشان داد که هر دو پروتئین p0 p0cy-rpv) و (p0cy-rps قادر به سرکوب خاموشی آر ان ای ایجاد شده توسط ترادف های تراژن سنس و تکرار معکوس در n. benthamiana هستند. نشان داده شد که هر دو پروتئین p0 می توانند تخریب پروتئین argonaute-1 را تسهیل کنند. علاوه بر این، تمایل م...
Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins.
Sterols and related membrane-perturbing agents are subject to a quality control cycle. Compounds that fail to pass this control are acetylated and secreted into the culture media, whereas lipids that pass the cycle are deacetylated and retained within the cell. Here we describe the identification of a family of conserved proteins, the Pathogen-Related Yeast (PRY) proteins, as a class of sterol-...
متن کاملGeneral RNA binding proteins render translation cap dependent.
Translation in rabbit reticulocyte lysate is relatively independent of the presence of the mRNA m7G cap structure and the cap binding protein, eIF-4E. In addition, initiation occurs frequently at spurious internal sites. Here we show that a critical parameter which contributes to cap-dependent translation is the amount of general RNA binding proteins in the extract. Addition of several general ...
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ژورنال
عنوان ژورنال: Translation
سال: 2016
ISSN: 2169-0731
DOI: 10.1080/21690731.2016.1257408